By Yutaka Kawarabayasi Ph.D. (auth.), Pratyoosh Shukla, Brett I. Pletschke (eds.)
Enzyme know-how is one the main promising disciplines in smooth biotechnology. during this booklet, the functions of a wide selection of enzymes are highlighted. present experiences in enzyme know-how are concentrated in the direction of the invention of novel enzymes (termed “bio-discovery” or “bio-prospecting”) and the identity and elucidation of novel pathways of those novel enzymes with emphasis on their commercial relevance. With the advance of molecular strategies and different bioinformatics instruments, the time to combine this topic with different fields within the existence sciences has arrived. A swift growth of the information base within the box of enzyme biotechnology has happened during the last few years. a lot of this growth has been pushed by way of the bio-discovery of many new enzymes from quite a lot of environments, a few severe in nature, through next protein (enzyme) engineering. those enzymes have discovered quite a lot of functions, starting from bioremediation, bio-monitoring, biosensor improvement, bioconversion to biofuels and different biotechnologically very important value-added items. Hydrolases represent a tremendous part of the worldwide annual profit generated through commercial enzymes and the emphasis has accordingly been put on those enzymes and their functions. With the significant curiosity of researchers lively during this region, this ebook will serve to supply details on present facets during this box of research. within the present variation, the contributions of many varied issues in the direction of constructing new instructions of analysis within the region of enzyme biotechnology are defined. This e-book serves to supply a different resource of data to undergraduates, put up graduates and doctoral classes in microbiology and biotechnology in addition to allied lifestyles sciences. the current variation of the booklet covers all very important parts of enzyme biotechnology i.e. the wide range of enzymes within the box of enzyme biotechnology and their commercial purposes, new equipment and cutting-edge details on glossy tools of enzyme discovery. This e-book will act nearly as good source on lots of the present features of enzyme know-how for all scholars engaged in bioengineering and biotechnology.
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Extra info for Advances in Enzyme Biotechnology
J. es M. de Abreu • M. Fernandez-Lobato Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Madrid 28049, Spain P. Urrutia Instituto de Catálisis y Petroleoquímica, CSIC, Cantoblanco, Marie Curie 2, Madrid 28049, Spain School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile P. I. 1007/978-81-322-1094-8_3, © Springer India 2013 23 B. Rodriguez-Colinas et al. 23), also called lactases, catalyze the hydrolysis of the galactosyl moiety from the nonreducing end of various oligosaccharides.
Different methods of permeabilizing cells that do not significantly affect enzymatic activity have been described, including drying, treatment with solvents or surfactants, lyophilization, ultrasonic treatment, and mechanical disruption (Panesar et al. 2007, 2011). In our work, a double treatment (ethanol incubation and lyophilization) was applied to harvested K. lactis CECT 1,931 cells. Although cells treated with short-chain alcohols may die in many cases, intracellular enzymes may resist such treatment and, in consequence, are not inactivated.
2008). Several approaches for immobilization of this enzyme have been proposed using different carriers (Maugard et al. 2003; Zhou et al. 2003; Zhou and Chen 2001). Recently, the crystallization and resolution of its three-dimensional structure has been reported (Pereira-Rodriguez et al. 2010). We studied in detail the transgalactosylation activity of K. lactis β-galactosidase. Different enzyme preparations were assayed: (1) ethanolpermeabilized cells of a strain of K. lactis and (2) two soluble β-galactosidases from K.
Advances in Enzyme Biotechnology by Yutaka Kawarabayasi Ph.D. (auth.), Pratyoosh Shukla, Brett I. Pletschke (eds.)