By Gal Bitan, David B. Teplow (auth.), Einar M. Sigurdsson (eds.)
A confirmed selection of simply reproducible innovations for learning amyloid proteins and their involvement within the etiology, pathogenesis, analysis, and treatment of amyloid illnesses. The participants offer tools for the education of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical innovations for his or her examine, and mobilephone tradition types and assays for the creation of amyloid proteins. extra chapters current comfortably reproducible strategies for amyloid extraction from tissue, its detection in vitro and in vivo, in addition to nontransgenic tools for constructing amyloid mouse versions. The protocols stick to the winning tools in Molecular Biology™ sequence structure, every one supplying step by step laboratory directions, an advent outlining the primary in the back of the approach, lists of the required apparatus and reagents, and tips about troubleshooting and fending off recognized pitfalls.
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Additional resources for Amyloid Proteins: Methods and Protocols
Et al. (1997) Mutations in the transmembrane domain of APP altering gamma-secretase specificity. Biochemistry 36(49), 15396–15403. , et al. (2000) A beta peptide immunization reduces behavioural impairment and plaques in a model of Alzheimer’s disease. Nature 408(6815), 979–982. , et al. (2000) A beta peptide vaccination prevents memory loss in an animal model of Alzheimer’s disease. Nature 408(6815), 982–985. , et al. (2001) The ‘Arctic’ APP mutation (E693G) causes Alzheimer’s disease by enhanced Abeta protofibril formation.
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We would like to thank Drs. Peter T. Lansbury, Tomas Ding, and Mary Jo LaDu for providing the images used in Figs. 1 and 4. Also, we would like to thank Dr. Dean Hartley for assistance with the Aβ solubilization protocols. Support was also derived from the NIH (AG08470). References 1. 1 Kelly, J. W. (1998) The alternative conformations of amyloidogenic proteins and their multi-step assembly pathways. Curr. Opin. Struct. Biol. 8(1), 101–106. 2. 2 Rochet, J. C. and Lansbury, P. T. Jr. (2000) Amyloid fibrillogenesis: themes and variations.
Amyloid Proteins: Methods and Protocols by Gal Bitan, David B. Teplow (auth.), Einar M. Sigurdsson (eds.)